diff --git a/src/System.Runtime.Loader/tests/AssemblyLoadContextTest.cs b/src/System.Runtime.Loader/tests/AssemblyLoadContextTest.cs
index 4f43e086fad8..330c0e98af25 100644
--- a/src/System.Runtime.Loader/tests/AssemblyLoadContextTest.cs
+++ b/src/System.Runtime.Loader/tests/AssemblyLoadContextTest.cs
@@ -145,9 +145,7 @@ public static void DefaultAssemblyLoadContext_Properties()
             Assert.Contains("\"Default\"", alc.ToString());
             Assert.Contains("System.Runtime.Loader.DefaultAssemblyLoadContext", alc.ToString());
             Assert.Contains(alc, AssemblyLoadContext.All);
-#if CoreCLR_23583
             Assert.Contains(Assembly.GetCallingAssembly(), alc.Assemblies);
-#endif
         }
 
         [Fact]
@@ -161,9 +159,7 @@ public static void PublicConstructor_Default()
             Assert.Contains("PublicConstructor", alc.ToString());
             Assert.Contains("System.Runtime.Loader.AssemblyLoadContext", alc.ToString());
             Assert.Contains(alc, AssemblyLoadContext.All);
-#if CoreCLR_23583
             Assert.Empty(alc.Assemblies);
-#endif
         }
 
         [Theory]
@@ -179,9 +175,7 @@ public static void PublicConstructor_Theory(string name, bool isCollectible)
             Assert.Contains(name, alc.ToString());
             Assert.Contains("System.Runtime.Loader.AssemblyLoadContext", alc.ToString());
             Assert.Contains(alc, AssemblyLoadContext.All);
-#if CoreCLR_23583
             Assert.Empty(alc.Assemblies);
-#endif
         }
     }
 }
diff --git a/src/System.Runtime.Loader/tests/DefaultContext/DefaultLoadContextTest.cs b/src/System.Runtime.Loader/tests/DefaultContext/DefaultLoadContextTest.cs
index 55f4b709b8dd..13aae6f8a4f4 100644
--- a/src/System.Runtime.Loader/tests/DefaultContext/DefaultLoadContextTest.cs
+++ b/src/System.Runtime.Loader/tests/DefaultContext/DefaultLoadContextTest.cs
@@ -104,9 +104,7 @@ public void LoadInDefaultContext()
 
             // We should have successfully loaded the assembly in secondary load context.
             Assert.NotNull(slcLoadedAssembly);
-#if CoreCLR_23583
             Assert.Contains(slcLoadedAssembly, slc.Assemblies);
-#endif
 
             // And make sure the simple name matches
             Assert.Equal(TestAssemblyName, slcLoadedAssembly.GetName().Name);
@@ -129,9 +127,7 @@ public void LoadInDefaultContext()
 
             // We should have successfully loaded the assembly in default context.
             Assert.NotNull(assemblyExpectedFromLoad);
-#if CoreCLR_23583
             Assert.Contains(assemblyExpectedFromLoad, AssemblyLoadContext.Default.Assemblies);
-#endif
 
             // We should have only invoked non-Null returning handler once
             Assert.Equal(1, _numNonNullResolutions);
diff --git a/src/System.Runtime/tests/System/Reflection/IsCollectibleTests.cs b/src/System.Runtime/tests/System/Reflection/IsCollectibleTests.cs
index 960a371d3a6b..25033169a153 100644
--- a/src/System.Runtime/tests/System/Reflection/IsCollectibleTests.cs
+++ b/src/System.Runtime/tests/System/Reflection/IsCollectibleTests.cs
@@ -53,9 +53,7 @@ public void Assembly_IsCollectibleFalse_WhenUsingAssemblyLoad()
                 Assert.Contains("\"Default\"", alc.ToString());
                 Assert.Contains("System.Runtime.Loader.DefaultAssemblyLoadContext", alc.ToString());
                 Assert.Contains(alc, AssemblyLoadContext.All);
-#if CoreCLR_23583
                 Assert.Contains(asm, alc.Assemblies);
-#endif
 
                 return RemoteExecutor.SuccessExitCode;
             }).Dispose();
@@ -78,9 +76,7 @@ public void Assembly_IsCollectibleFalse_WhenUsingAssemblyLoadContext()
                 Assert.Contains("Assembly_IsCollectibleFalse_WhenUsingAssemblyLoadContext", alc.ToString());
                 Assert.Contains("System.Runtime.Loader.AssemblyLoadContext", alc.ToString());
                 Assert.Contains(alc, AssemblyLoadContext.All);
-#if CoreCLR_23583
                 Assert.Contains(asm, alc.Assemblies);
-#endif
 
                 return RemoteExecutor.SuccessExitCode;
             }).Dispose();
@@ -103,9 +99,7 @@ public void Assembly_IsCollectibleTrue_WhenUsingTestAssemblyLoadContext()
                 Assert.Contains("\"\"", alc.ToString());
                 Assert.Contains("System.Reflection.Tests.TestAssemblyLoadContext", alc.ToString());
                 Assert.Contains(alc, AssemblyLoadContext.All);
-#if CoreCLR_23583
                 Assert.Contains(asm, alc.Assemblies);
-#endif
 
                 return RemoteExecutor.SuccessExitCode;
             }).Dispose();